Porous PEDOT:PSS Particles and their Application as Tunable Cell Culture Substrate

Due to its biocompatibility, electrical conductivity, and tissue-like elasticity, poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) constitutes a highly promising material regarding the fabrication of smart cell culture substrates. However, until now, high-throughput synthesis pure PEDOT:PSS geometries was restricted flat sheets fibers. In this publication, first microfluidic process for spherical, porous, particles adjustable properties is presented. The are synthesized by generation emulsion droplets within 1-octanol continuous phase their subsequent coagulation partial crystallization in an isopropanol (IPA)/sulfuric acid (SA) bath. allows tailor central particle characteristics such as crystallinity, diameter, pore size well electrochemical mechanical simply adjusting IPA:SA ratio during droplet coagulation. To demonstrate applicability potential substrate, cultivations L929 mouse fibroblast cells MRC-5 human conducted. Both lines present exponential growth on reach confluency with viabilities above 95 vol.% day 9. Single analysis could moreover reveal that mechanotransduction infiltration can be controlled adjustment crystallinity.